A Round-bottom 96-well Polystyrene Plate Coated with 2-methacryloyloxyethyl Phosphorylcholine as an Effective Tool for Embryoid Body Formation
نویسندگان
چکیده
منابع مشابه
Thiolated 2-methacryloyloxyethyl phosphorylcholine for an antifouling biosensor platform.
We developed a new building block for a protein- and cell-repellant self-assembled monolayer (SAM) from 2-methacryloyloxyethyl phosphorylcholine (MPC) via a simple Michael-type addition to one mercapto group in alkanedithiol. The thiolated MPC can enable functionalization of a noble metal electrode to minimize noise signal in biosensing.
متن کاملEvaluation of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer-coated dressing on surgical wounds.
The ideal dressing material is bio-inert and keeps the wound site moist. It is equally important that no regenerative tissue is peeled off on the removal of the dressing. 2-Methacryloyloxyethyl phosphorylcholine (MPC) has a phospholipid polar group that mimics a biomembrane. We prepared poly [MPC-co-n-dodecyl methacrylate (DMA)] (PMD), using conventional radical polymerization with 2,2'-azobisi...
متن کاملAn Automated 96-Well-Plate Loader for the FACScan
Staining multiple samples for data acquisition with a flow cytometer is done in 96-well plates to save time and make large data assessment in one working day possible. However, the inability of the FACScan R © to take up the samples from 96well plates is a major drawback. In order to avoid the individual transfer of samples to tubes, we have developed a system, which allows using the FACScan R ...
متن کاملA 96-Well Plate Assay for Relative Monoclonal Antibody Titers
LEVEL: INTERMEDIATE M onoclonal antibodies are used to treat a wide range of illnesses such as rheumatoid arthritis, Crohn’s Disease, and follicular non-Hodgkin lymphoma (1). The identification and use of certain growth medium additives and modified culture conditions during cell culture processes that produce MAbs may lower production costs—which could help lower the overall costs of those dru...
متن کاملEffective embryoid body formation from induced pluripotent stem cells for regeneration of respiratory epithelium.
OBJECTIVES/HYPOTHESIS We have previously demonstrated the potential use of induced pluripotent stem (iPS) cells for regeneration of respiratory epithelium by culturing embryoid bodies (EBs). The aim of the present study was to determine the most effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium. STUDY DESIGN Experimental study. METHODS iPS cells...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytotechnology
سال: 2005
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-005-3743-x